Nasal Wipes for Influenza A Virus Detection and Isolation from Swine.
Identifieur interne : 000D19 ( Main/Exploration ); précédent : 000D18; suivant : 000D20Nasal Wipes for Influenza A Virus Detection and Isolation from Swine.
Auteurs : Jacqueline M. Nolting ; Christine M. Szablewski ; Jody L. Edwards ; Sarah W. Nelson ; Andrew S. Bowman [États-Unis]Source :
- Journal of visualized experiments : JoVE [ 1940-087X ] ; 2015.
Descripteurs français
- KwdFr :
- Animaux, Bandelettes réactives, Infections à Orthomyxoviridae (médecine vétérinaire), Infections à Orthomyxoviridae (virologie), Maladies des porcs (virologie), Sous-type H1N1 du virus de la grippe A (isolement et purification), Sous-type H3N2 du virus de la grippe A (isolement et purification), Suidae, Virus de la grippe A (isolement et purification).
- MESH :
- isolement et purification : Sous-type H1N1 du virus de la grippe A, Sous-type H3N2 du virus de la grippe A, Virus de la grippe A.
- médecine vétérinaire : Infections à Orthomyxoviridae.
- virologie : Infections à Orthomyxoviridae, Maladies des porcs.
- Animaux, Bandelettes réactives, Suidae.
English descriptors
- KwdEn :
- Animals, Influenza A Virus, H1N1 Subtype (isolation & purification), Influenza A Virus, H3N2 Subtype (isolation & purification), Influenza A virus (isolation & purification), Orthomyxoviridae Infections (veterinary), Orthomyxoviridae Infections (virology), Reagent Strips, Swine, Swine Diseases (virology).
- MESH :
- chemical : Reagent Strips.
- isolation & purification : Influenza A Virus, H1N1 Subtype, Influenza A Virus, H3N2 Subtype, Influenza A virus.
- veterinary : Orthomyxoviridae Infections.
- virology : Orthomyxoviridae Infections, Swine Diseases.
- Animals, Swine.
Abstract
Surveillance for influenza A viruses in swine is critical to human and animal health because influenza A virus rapidly evolves in swine populations and new strains are continually emerging. Swine are able to be infected by diverse lineages of influenza A virus making them important hosts for the emergence and maintenance of novel influenza A virus strains. Sampling pigs in diverse settings such as commercial swine farms, agricultural fairs, and live animal markets is important to provide a comprehensive view of currently circulating IAV strains. The current gold-standard ante-mortem sampling technique (i.e. collection of nasal swabs) is labor intensive because it requires physical restraint of the pigs. Nasal wipes involve rubbing a piece of fabric across the snout of the pig with minimal to no restraint of the animal. The nasal wipe procedure is simple to perform and does not require personnel with professional veterinary or animal handling training. While slightly less sensitive than nasal swabs, virus detection and isolation rates are adequate to make nasal wipes a viable alternative for sampling individual pigs when low stress sampling methods are required. The proceeding protocol outlines the steps needed to collect a viable nasal wipe from an individual pig.
DOI: 10.3791/53313
PubMed: 26709840
Affiliations:
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Le document en format XML
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Nolting</name><affiliation><nlm:affiliation>Department of Veterinary Preventive Medicine, The Ohio State University.</nlm:affiliation><wicri:noCountry code="subField">The Ohio State University</wicri:noCountry></affiliation></author><author><name sortKey="Szablewski, Christine M" sort="Szablewski, Christine M" uniqKey="Szablewski C" first="Christine M" last="Szablewski">Christine M. Szablewski</name><affiliation><nlm:affiliation>Department of Veterinary Preventive Medicine, The Ohio State University.</nlm:affiliation><wicri:noCountry code="subField">The Ohio State University</wicri:noCountry></affiliation></author><author><name sortKey="Edwards, Jody L" sort="Edwards, Jody L" uniqKey="Edwards J" first="Jody L" last="Edwards">Jody L. Edwards</name><affiliation><nlm:affiliation>Department of Veterinary Preventive Medicine, The Ohio State University.</nlm:affiliation><wicri:noCountry code="subField">The Ohio State University</wicri:noCountry></affiliation></author><author><name sortKey="Nelson, Sarah W" sort="Nelson, Sarah W" uniqKey="Nelson S" first="Sarah W" last="Nelson">Sarah W. Nelson</name><affiliation><nlm:affiliation>Department of Veterinary Preventive Medicine, The Ohio State University.</nlm:affiliation><wicri:noCountry code="subField">The Ohio State University</wicri:noCountry></affiliation></author><author><name sortKey="Bowman, Andrew S" sort="Bowman, Andrew S" uniqKey="Bowman A" first="Andrew S" last="Bowman">Andrew S. Bowman</name><affiliation wicri:level="1"><nlm:affiliation>Department of Veterinary Preventive Medicine, The Ohio State University; bowman.214@osu.edu.</nlm:affiliation><country wicri:rule="url">États-Unis</country><wicri:regionArea>Department of Veterinary Preventive Medicine</wicri:regionArea></affiliation></author></analytic><series><title level="j">Journal of visualized experiments : JoVE</title><idno type="eISSN">1940-087X</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Influenza A Virus, H1N1 Subtype (isolation & purification)</term><term>Influenza A Virus, H3N2 Subtype (isolation & purification)</term><term>Influenza A virus (isolation & purification)</term><term>Orthomyxoviridae Infections (veterinary)</term><term>Orthomyxoviridae Infections (virology)</term><term>Reagent Strips</term><term>Swine</term><term>Swine Diseases (virology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Bandelettes réactives</term><term>Infections à Orthomyxoviridae (médecine vétérinaire)</term><term>Infections à Orthomyxoviridae (virologie)</term><term>Maladies des porcs (virologie)</term><term>Sous-type H1N1 du virus de la grippe A (isolement et purification)</term><term>Sous-type H3N2 du virus de la grippe A (isolement et purification)</term><term>Suidae</term><term>Virus de la grippe A (isolement et purification)</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Reagent Strips</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Influenza A Virus, H1N1 Subtype</term><term>Influenza A Virus, H3N2 Subtype</term><term>Influenza A virus</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Sous-type H1N1 du virus de la grippe A</term><term>Sous-type H3N2 du virus de la grippe A</term><term>Virus de la grippe A</term></keywords><keywords scheme="MESH" qualifier="médecine vétérinaire" xml:lang="fr"><term>Infections à Orthomyxoviridae</term></keywords><keywords scheme="MESH" qualifier="veterinary" xml:lang="en"><term>Orthomyxoviridae Infections</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Infections à Orthomyxoviridae</term><term>Maladies des porcs</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Orthomyxoviridae Infections</term><term>Swine Diseases</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Swine</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Bandelettes réactives</term><term>Suidae</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Surveillance for influenza A viruses in swine is critical to human and animal health because influenza A virus rapidly evolves in swine populations and new strains are continually emerging. Swine are able to be infected by diverse lineages of influenza A virus making them important hosts for the emergence and maintenance of novel influenza A virus strains. Sampling pigs in diverse settings such as commercial swine farms, agricultural fairs, and live animal markets is important to provide a comprehensive view of currently circulating IAV strains. The current gold-standard ante-mortem sampling technique (i.e. collection of nasal swabs) is labor intensive because it requires physical restraint of the pigs. Nasal wipes involve rubbing a piece of fabric across the snout of the pig with minimal to no restraint of the animal. The nasal wipe procedure is simple to perform and does not require personnel with professional veterinary or animal handling training. While slightly less sensitive than nasal swabs, virus detection and isolation rates are adequate to make nasal wipes a viable alternative for sampling individual pigs when low stress sampling methods are required. The proceeding protocol outlines the steps needed to collect a viable nasal wipe from an individual pig. </div></front></TEI><affiliations><list><country><li>États-Unis</li></country></list><tree><noCountry><name sortKey="Edwards, Jody L" sort="Edwards, Jody L" uniqKey="Edwards J" first="Jody L" last="Edwards">Jody L. Edwards</name><name sortKey="Nelson, Sarah W" sort="Nelson, Sarah W" uniqKey="Nelson S" first="Sarah W" last="Nelson">Sarah W. Nelson</name><name sortKey="Nolting, Jacqueline M" sort="Nolting, Jacqueline M" uniqKey="Nolting J" first="Jacqueline M" last="Nolting">Jacqueline M. Nolting</name><name sortKey="Szablewski, Christine M" sort="Szablewski, Christine M" uniqKey="Szablewski C" first="Christine M" last="Szablewski">Christine M. Szablewski</name></noCountry><country name="États-Unis"><noRegion><name sortKey="Bowman, Andrew S" sort="Bowman, Andrew S" uniqKey="Bowman A" first="Andrew S" last="Bowman">Andrew S. 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